Enhanced microbial lipid production under two-stage halo-alkaliphilic co-culture conditions by Phaeodactylum tricornutum and Cryptococcus curvatus
이 페이지는 아래 학술 논문의 초록(Abstract) 전문을 제공합니다. 원문은 하단 링크에서 확인하세요. ◆ 논문 초록 (Abstract) Microbial lipids are promising bioenergy feedstocks, but limited by low yields, high substrate and sterilization costs,...
이 페이지는 아래 학술 논문의 초록(Abstract) 전문을 제공합니다. 원문은 하단 링크에서 확인하세요.
◆ 논문 초록 (Abstract)
Microbial lipids are promising bioenergy feedstocks, but limited by low yields, high substrate and sterilization costs, dissolved oxygen (DO) limitations, and poor tolerance to lignocellulosic biomass (LCB) inhibitors. Here, we develop a two-stage halo-alkaliphilic co-culture of the alga Phaeodactylum tricornutum and yeast Cryptococcus curvatus that integrates multiple industrially relevant constraints within a single process framework, enabling robust lipid production under seawater-based non-sterile cultivation, reduced oxygen transfer (OT) conditions, high salinity and pH, and LCB-derived phenolic inhibition. Under two-stage cultivation, the co-culture at a strains inoculum ratio of 1:1 (w/w) produced 7.2 g/L lipid, 46.4% lipid content and a lipid yield of 0.242 g/g glucose, indicating a 2.9-fold (189%) increase in lipid titer compared with the combined lipid titer of both monocultures under the same conditions. Similarly, the co-culture at a strains inoculum ratio of 1:1 maintained robust lipid production under reduced OT conditions, producing 5.0 g/L lipid compared with the sum of both monocultures (1.8 g/L), corresponding to a 2.8-fold (178%) increase, demonstrating the robustness of the co-culture system. Moreover, the co-culture enabled non-sterile lipid production in seawater-based media at pH 10. On synthetic LCB hydrolysates containing 1.1 g/L vanillin, the two-stage co-culture with a strains inoculum ratio of 1:1 produced up to 5.2 g/L lipid with 44.8% lipid content, corresponding to a 4.5-fold (354%) increase in lipid titer over the sum of both monocultures. The co-culture improved sugar consumption, buffered culture, and tolerated high salt, pH and vanillin stress. These results demonstrated a step toward next-generation microbial lipid biorefinery.
◆ 원문 정보
저자: Chao H, Kamal R, Luo J, Yi J, Hu Z et al.
저널: Bioresour Technol
연도: 2026
DOI: 10.1016/j.biortech.2026.134407
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