[Effect of electroacupuncture of “Neiguan” (PC6) and “Shenmen” (HT7) on cardiac function and neovascularization in rats with chronic heart failure based on Notch signaling pathway]
이 페이지는 아래 학술 논문의 초록(Abstract) 전문을 제공합니다. 원문은 하단 링크에서 확인하세요. ◆ 논문 초록 (Abstract) OBJECTIVES: To observe the effect of electroacupuncture (EA) on the activities of N-terminal B-type natriuretic peptide...
이 페이지는 아래 학술 논문의 초록(Abstract) 전문을 제공합니다. 원문은 하단 링크에서 확인하세요.
◆ 논문 초록 (Abstract)
OBJECTIVES: To observe the effect of electroacupuncture (EA) on the activities of N-terminal B-type natriuretic peptide (NT-proBNP)/vascular endothelial growth factor (VEGF)/neurogenic site-gap homologous protein (Notch) signaling pathway and related factors in rats with chronic heart failure (CHF), so as to explore its regulatory mechanism underlying improvement of CHF. METHODS: Male SD rats were randomly divided into sham-operation, CHF model, EA and EA+inhibitor [DAPT (GSI-IX), a γ-secretase inhibitor] groups (n=6 in each group). The CHF model was established by ligating the anterior descending branch of the left coronary artery. EA (2 Hz, an electric current intensity to induce slight shaking of the inserted acupuncture needles) was applied to bilateral “Neiguan”(PC6) and “Shenmen”(HT7) for 30 min, once daily, for 7 d. Rats of the EA+inhibitor group received intraperitoneal injection of DAPT (3 mg/kg) 30 min before each EA intervention. A small animal color Doppler ultrasound machine was used to collect the left ventricular ejection fraction (LVEF), left ventricular fractional shortening rate (LVFS), left ventricular end-systolic diameter (LVIDs) for evaluating the left ventricle function. Histopathological changes of the cardiac tissue were observed by H.E. staining and Masson staining. The contents of serum NT-proBNP and VEGF were detected by using ELISA. The microvascular density (MVD) of the left ventricular myocardium was observed by CD34 immunohistochemistry. The expression levels of myocardial Notch signaling pathway-related proteins Notch1 and Delta-like 4 ligand (DLL4) were detected by using Western blot. RESULTS: Compared with the sham operation group, the model group had a significant increase in the LVIDs, serum NF-proBNP content, MVD and expression levels of Notch1 and DLL4 protein in the left ventricular myocardial tissue (P<0.000 1, P<0.001, P<0.01), and a significant decrease in the LVEF, LVFS and serum VEGF content (P<0.000 1). In comparison with the model group, the EA group had a significant decrease in the LVIDs and serum NF-proBNP content(P<0.001), and a significant increase in the LVEF, LVFS and serum VEGF content (P<0.001, P<0.000 1), while the MVD and expression levels of Notch1 and DLL4 protein in the left ventricular myocardial tissue were more increased (P<0.001, P<0.05, P<0.01). In comparison with the EA group, both the striking increase and decrease of the indexes mentioned above were all reversed in the EA+inhibitor group (P<0.05, P<0.01, P<0.000 1, P<0.001) except LVIDs. Histopathological observation showed obvious myocardial fiber rupture, irregular arrangement, cell necrosis, unclear nucleoli, fibrous hyperplasia, deposition of collagenous fibers, and a large number of inflammatory infiltration in the myocardial tissue of the model group, which was relatively milder in the EA group but not in the EA+inhibitor group. CONCLUSIONS: EA of PC6 and HT7 can improve the cardiac function of rats with CHF, which may be associated with its functions in promoting the neovascularization of ischemic myocardium, reducing ischemic injury, and protecting myocardial tissue structure via up-regulation of the serum VEGF content and the expressions of Notch1 and DLL4 protein. 目的: 观察电针“内关”“神门”对慢性心力衰竭（CHF）大鼠N末端B型利钠肽原（NT-proBNP）/血管内皮生长因子（VEGF）/神经源性位点缺口同源蛋白（Notch）信号通路及相关因子的影响，探讨电针对心脏微血管新生的调控机制。方法: SD大鼠随机分为假手术组、模型组、电针组、电针+抑制剂组，每组6只。采用结扎冠状动脉左前降支法制备CHF大鼠模型。电针组予双侧“内关”“神门”电针治疗，30 min/次，1次/d;电针+抑制剂组在电针前30 min腹腔注射γ-分泌酶抑制剂（DAPT，3 mg/kg），1次/d。以上各组均连续治疗7 d。采用小动物超声心动图检测大鼠心功能;HE染色、Masson染色观察大鼠心脏病理形态;ELISA法检测大鼠血清中NT-proBNP、VEGF含量;免疫组织化学染色观察心肌微血管数量（MVD）;Western blot法检测心肌组织Notch信号通路相关蛋白Notch1、Delta样4配体（DLL4）的表达。结果: 与假手术组相比，模型组大鼠左心室射血分数（LVEF）值、左心室短轴缩短率（LVFS）值均降低（P<0.000 1），左心室收缩末期内径（LVIDs）值升高（P<0.000 1），血清NT-proBNP含量升高（P<0.001）、VEGF含量降低（P<0.000 1），MVD增加（P<0.01），Notch1、DLL4的蛋白表达水平升高（P<0.001，P<0.000 1）;心肌组织呈缺血样损伤，大量胶原纤维沉积。与模型组相比，电针组大鼠LVEF、LVFS值均升高（P<0.001）、LVIDs值降低（P<0.001），血清NT-proBNP含量降低（P<0.001）、VEGF含量升高（P<0.000 1），MVD增加（P<0.001），Notch1、DLL4蛋白表达水平升高（P<0.05，P<0.01）;心肌病理形态和纤维化程度得到改善。与电针组比较，电针+抑制剂组大鼠LVEF、LVFS值降低（P<0.05），血清NT-proBNP含量升高（P<0.01）、VEGF含量降低（P<0.000 1），MVD减少（P<0.01），Notch1、DLL4蛋白表达水平降低（P<0.01，P<0.001）;心肌病理形态改变和纤维化程度均高于电针组。结论: 电针“内关”“神门”可通过促进缺血梗死区血管新生，减轻缺血性损伤，保护心肌组织，改善CHF大鼠的心功能，其机制可能与调控Notch 信号通路，增加相关因子的表达有关。.
◆ 원문 정보
저자: Huang JZ, Zeng QL, Guo XN, Wu HS, Zhang JJ et al.
저널: Zhen Ci Yan Jiu
연도: 2026
DOI: 10.13702/j.1000-0607.20241251
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